1CYX

QUINOL OXIDASE (PERIPLASMIC FRAGMENT OF SUBUNIT II WITH ENGINEERED CU-A BINDING SITE)(CYOA)

Experimental procedure

Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	1992
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	46.000, 98.200, 39.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.300
R-factor	0.196
Rwork	0.196
R-free	0.31600
RMSD bond length	0.010
RMSD bond angle	26.270 *
Data reduction software	XDS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	1000.000
High resolution limit [Å]	2.300
Rmerge	0.084 *
Total number of observations	17363 *
Number of reflections	7426 *
Completeness [%]	87.3
Redundancy	3.19

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.2 *	4 *	pH 4.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15-20 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)
3	1	reservoir	PEG6000	20 (%(w/v))
4	1	reservoir	acetic acid	180 (mM)
5	1	reservoir	sodium acetate	20 (mM)
6	1	reservoir	ammonium sulfate	50 (mM)
7	1	reservoir		1 (mM)