1CX4

CRYSTAL STRUCTURE OF A DELETION MUTANT OF THE TYPE II BETA REGULATORY SUBUNIT OF CAMP-DEPENDENT PROTEIN KINASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	300.3
Detector technology	IMAGE PLATE
Collection date	1999-01-06
Detector	MARRESEARCH
Spacegroup name	P 61
Unit cell lengths	161.620, 161.620, 39.660
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.000 - 2.450
R-factor	0.176
Rwork	0.176
R-free	0.19800
RMSD bond length	0.006
RMSD bond angle	24.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.560
High resolution limit [Å]	2.450	2.450
Rmerge	0.052	0.325
Number of reflections	21965
<I/σ(I)>	14.3	3.94
Completeness [%]	98.7	97.9
Redundancy	4.182	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.75		Diller, T.C., (2000) Protein Expression Purif., 20, 357. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	26 (mg/ml)
2	1	reservoir	Tris	80 (mM)	pH7.75
3	1	reservoir	PEG3400	29 (%)
4	1	reservoir		80 (mM)