1CW0
CRYSTAL STRUCTURE ANALYSIS OF VERY SHORT PATCH REPAIR (VSR) ENDONUCLEASE IN COMPLEX WITH A DUPLEX DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL24XU |
| Synchrotron site | SPring-8 |
| Beamline | BL24XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-06-10 |
| Detector | RIGAKU RAXIS |
| Spacegroup name | P 65 |
| Unit cell lengths | 63.000, 63.000, 152.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.000 - 2.300 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.23000 |
| Starting model (for MR) | TRUNCATED VSR MODEL 1vsr |
| RMSD bond length | 0.011 |
| RMSD bond angle | 24.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.000 * | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.048 * | 0.261 * |
| Number of reflections | 15168 * | |
| <I/σ(I)> | 14.4 | |
| Completeness [%] | 99.4 * | 100 |
| Redundancy | 4.6 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 5.6 | 20 * | pH 5.60, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | DNA | 1.5 (mM) | |
| 2 | 1 | 1 | protein | 0.5 (mM) | |
| 3 | 1 | 1 | 20 (mM) | ||
| 4 | 1 | 1 | PEG8000 | 2.5 (%) | |
| 5 | 1 | 1 | MES | 25 (mM) | |
| 6 | 1 | 1 | 100 (mM) | ||
| 7 | 1 | 1 | 15 (mM) | ||
| 8 | 1 | 1 | Tris-HCl | 6.5 (mM) | |
| 9 | 1 | 1 | 31.0 (mM) |
