1CVL
CRYSTAL STRUCTURE OF BACTERIAL LIPASE FROM CHROMOBACTERIUM VISCOSUM ATCC 6918
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-11 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 41.080, 156.820, 43.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.600 |
| R-factor | 0.178 * |
| Rwork | 0.178 |
| Structure solution method | MIR |
| RMSD bond length | 0.021 |
| RMSD bond angle | 0.044 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | MLPHARE |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.053 | 0.220 * |
| Total number of observations | 87722 * | |
| Number of reflections | 33644 | |
| <I/σ(I)> | 8.5 | 3.1 |
| Completeness [%] | 88.0 | 73.7 |
| Redundancy | 2.6 | 1.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6.4 | 292 * | PROTEIN WAS CRYSTALLIZED FROM 10-14 % PEG 4000, 10-14 % MPD, 100 MM CITRATE/PHOSPHATE BUFFER, PH 6.4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | lipase | 15 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 5 (mM) | |
| 3 | 1 | drop | n-octyl-beta-D-glycopyranoside | 0.25 (%(w/v)) | |
| 4 | 1 | reservoir | PEG4000 | 14 (%(w/v)) | |
| 5 | 1 | reservoir | MPD | 10-14 (%(v/v)) | |
| 6 | 1 | reservoir | citrate-phosphate | 100 (mM) |
