1CV7
Crystal structure of enhanced cyan-emission variant of GFP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-06-06 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.052, 62.781, 66.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.500 - 2.500 |
| R-factor | 0.1861 |
| Rwork | 0.186 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.400 |
| Data scaling software | SCALEPACK |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.500 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.121 | 0.611 |
| Number of reflections | 7829 | |
| <I/σ(I)> | 2.93 | |
| Completeness [%] | 97.4 | 95.5 |
| Redundancy | 6.9 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 12% PEG-1550, 50mM calcium acetate and 100 mM acetaete buffer, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
