1CUW
CUTINASE, G82A, A85F, V184I, A185L, L189F MUTANT
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Detector | MAR scanner 180 mm plate |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 130.400, 54.700, 69.840 |
Unit cell angles | 90.00, 116.10, 90.00 |
Refinement procedure
Resolution | 6.000 - 2.700 |
R-factor | 0.141 * |
Rwork | 0.142 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | MARXDS |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.700 * |
Rmerge | 0.158 |
Total number of observations | 23137 * |
Number of reflections | 9151 |
Completeness [%] | 94.0 |
Redundancy | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | PEG6000 | 2-20 (%(w/v)) | |
2 | 1 | drop | HEPES | 100 (mM) | |
3 | 1 | drop | cutinase | 5-15 (mg/ml) | |
4 | 1 | drop | MPD | 15 (%) | |
5 | 1 | reservoir | MPD | 15 (%) |