1CUD
CUTINASE, N172K, R196D MUTANT, MONOCLINIC CRYSTAL FORM WITH THREE MOLECULES PER ASYMMETRIC UNIT
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Detector | MAR scanner 180 mm plate |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 65.900, 73.300, 71.200 |
Unit cell angles | 90.00, 112.70, 90.00 |
Refinement procedure
Resolution | 6.000 - 2.700 |
R-factor | 0.151 * |
Rwork | 0.141 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.900 |
Data reduction software | MARXDS |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.700 * |
Rmerge | 0.173 |
Total number of observations | 79908 * |
Number of reflections | 16170 |
Completeness [%] | 93.0 |
Redundancy | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | PEG6000 | 2-20 (%(w/v)) | |
2 | 1 | drop | HEPES | 100 (mM) | |
3 | 1 | drop | cutinase | 5-15 (mg/ml) |