1CS7
SYNTHETIC DNA HAIRPIN WITH STILBENEDIETHER LINKER
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-02-06 |
| Detector | APS-1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 27.760, 67.890, 61.830 |
| Unit cell angles | 90.00, 96.80, 90.00 |
Refinement procedure
| Resolution | 8.000 - 3.200 |
| R-factor | 0.257 |
| Rwork | 0.257 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.710 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.260 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.097 | 0.095 |
| Number of reflections | 3434 | |
| Completeness [%] | 97.1 | 99 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 298 | OLIGONUCLEOTIDE, SRCL2, NA-CACODYLATE BUFFER, 2-METHYL-2,4-PENTANEDIOL, pH 6.3, VAPOR DIFFUSION, HANGING DROP at 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | SRCL2 | ||
| 2 | 1 | 1 | SODIUM CACODYLATE BUFFER | ||
| 3 | 1 | 1 | MPD | ||
| 4 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.8 (mM) | |
| 2 | 1 | drop | sodium cacodylate | 40 (mM) | |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | reservoir | 40 (%) |
