1CS3
STRUCTURE OF BTB/POZ TRANSCRIPTION REPRESSION DOMAIN FROM PROMELOCYTIC LEUKEMIA ZINC FINGER ONCOPROTEIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 190 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-10-03 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 0.9879,0.9795,0.9791, 0.9667 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 38.800, 78.200, 85.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.228 |
| Rwork | 0.228 |
| R-free | 0.27200 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.090 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.060 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.070 | |
| Total number of observations | 115110 * | |
| Number of reflections | 8790 | |
| <I/σ(I)> | 35.5 | 24 |
| Completeness [%] | 96.7 | 95 |
| Redundancy | 13 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 | reservoir contains two times the concentration of salts, buffer, and precipitating agent * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | isopropanol | 8 (%) | |
| 3 | 1 | drop | 600 (mM) | ||
| 4 | 1 | drop | Tris-HCl | 50 (mM) | |
| 5 | 1 | drop | HEPES | 50 (mM) |
