1CRZ

CRYSTAL STRUCTURE OF THE E. COLI TOLB PROTEIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	105
Detector technology	CCD
Collection date	1999-05-11
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	63.180, 40.500, 76.300
Unit cell angles	90.00, 110.88, 90.00

Refinement procedure

Resolution	20.000 - 1.950
R-factor	0.188
Rwork	0.238
R-free	0.23800
RMSD bond length	0.009
RMSD bond angle	25.800 *
Data reduction software	DENZO
Data scaling software	CCP4 ((SCALA))
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.060
High resolution limit [Å]	1.950	1.950
Rmerge	0.061	0.228
Total number of observations	360642 *
Number of reflections	25138
<I/σ(I)>	7.9
Completeness [%]	94.5	84.8
Redundancy	7.2	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.5	293	Bourveret, E., (1998) These et Doctrat. Universite Aix-Marseille II. *
1	unknown *	7.5	293	Bourveret, E., (1998) These et Doctrat. Universite Aix-Marseille II. *
1	unknown *	7.5	293	Bourveret, E., (1998) These et Doctrat. Universite Aix-Marseille II. *