1CRX
CRE RECOMBINASE/DNA COMPLEX REACTION INTERMEDIATE I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-10-15 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 107.700, 121.000, 180.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.400 |
| R-factor | 0.208 * |
| Rwork | 0.201 |
| R-free | 0.26300 |
| Structure solution method | MIR, MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.850 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.470 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.069 * | |
| Number of reflections | 42896 | |
| <I/σ(I)> | 20 | 10 |
| Completeness [%] | 93.0 | 87 |
| Redundancy | 5 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5 | 18 * | 100 MM ACETATE, PH 5 24% MPD 20 MM CACL2, pH 5.0 |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | ACETATE | ||
| 2 | 1 | 1 | CACL2 | ||
| 3 | 1 | 1 | MPD | ||
| 4 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium acetate | 50 (mM) | |
| 2 | 1 | drop | MPD | 12 (%) | |
| 3 | 1 | drop | 10 (mM) | ||
| 4 | 1 | drop | Cre | 0.050 (mM) | |
| 5 | 1 | drop | loxA | 0.075 (mM) | |
| 6 | 1 | reservoir | acetate | 100 (mM) | |
| 7 | 1 | reservoir | MPD | 24 (%) | |
| 8 | 1 | reservoir | 20 (mM) |
