1CRW

CRYSTAL STRUCTURE OF APO-GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE FROM PALINURUS VERSICOLOR AT 2.0A RESOLUTION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Detector technology	DIFFRACTOMETER
Collection date	1990-11-10
Detector	WEISSENBERG
Spacegroup name	C 1 2 1
Unit cell lengths	128.450, 99.840, 80.790
Unit cell angles	90.00, 115.14, 90.00

Refinement procedure

Resolution	6.000 - 2.000
R-factor	0.179 *
Rwork	0.165
R-free	0.22800 *
RMSD bond length	0.006 *
RMSD bond angle	1.260 *
Data reduction software	WEIS
Data scaling software	WEIS
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	8.000 *
High resolution limit [Å]	2.000 *	1.800
Rmerge	0.071
Total number of observations	136230 *
Number of reflections	45695 *
Completeness [%]	74.5 *
Redundancy	1.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.1	291	Song, S.Y., (1988) Sci. Chin., 3, 313. *