1CRK
MITOCHONDRIAL CREATINE KINASE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-18 |
| Temperature [K] | 277 |
| Detector technology | AREA DETECTOR |
| Collection date | 1993-08-27 |
| Detector | SIEMENS |
| Spacegroup name | P 42 |
| Unit cell lengths | 126.000, 126.000, 144.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 3.000 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.26400 |
| Structure solution method | SIR AND PHASEEXTENS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 3.500 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.114 | 0.397 |
| Number of reflections | 45021 | |
| <I/σ(I)> | 14.84 | 2.32 |
| Completeness [%] | 99.5 | 99 |
| Redundancy | 6 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.7 | pH 6.7 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG1000 | 17 (%) | |
| 10 | 1 | drop | beta-mercaptoethanol | 2 (mM) | |
| 11 | 1 | drop | Na2 EDTA | 0.2 (mM) | |
| 2 | 1 | reservoir | sodium phosphate | 25 (mM) | |
| 3 | 1 | reservoir | 1 (mM) | ||
| 4 | 1 | reservoir | beta-mercaptoethanol | 2 (mM) | |
| 5 | 1 | reservoir | Na2 EDTA | 0.2 (mM) | |
| 6 | 1 | drop | protein | 2.5 (mg/ml) | |
| 7 | 1 | drop | PEG1000 | 8.5 (%) | |
| 8 | 1 | drop | sodium phosphate | 25 (mM) | |
| 9 | 1 | drop | 1 (mM) |
