1CQR

CRYSTAL STRUCTURE OF THE STROMELYSIN CATALYTIC DOMAIN AT 2.0 A RESOLUTION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1996-05-24
Detector	RIGAKU RAXIS II
Spacegroup name	P 21 21 21
Unit cell lengths	38.200, 79.100, 107.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	12.000 - 2.000
R-factor	0.199
Rwork	0.199
R-free	0.26600
RMSD bond length	0.017
RMSD bond angle	3.210
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	12.000	12.000
High resolution limit [Å]	2.000	2.000
Rmerge	0.058	0.058
Number of reflections	21471
<I/σ(I)>	10.9
Completeness [%]	94.7	94.7
Redundancy	4.3	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *		298	20-24% PEG 8000, 2.5% 2-propanol, 10mM CaCl2, 0.1 M Tris-HCL buffer, VAPOR DIFFUSION, HANGING DROP, temperature 298.K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG8000	20-24 (%(w/v))
3	1	reservoir	2-propanol	2.5 (%(v/v))
4	1	reservoir		10 (mM)
5	1	reservoir	Tris-HCl	0.1 (M)