1CQJ
CRYSTAL STRUCTURE OF DEPHOSPHORYLATED E. COLI SUCCINYL-COA SYNTHETASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 283 |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-12-04 |
| Detector | FUJI |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 98.820, 98.820, 404.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.900 |
| R-factor | 0.178 |
| Rwork | 0.172 |
| R-free | 0.22800 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.900 |
| Data reduction software | WEIS |
| Data scaling software | SCALKB2 |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.092 | 0.350 |
| Total number of observations | 258739 * | |
| Number of reflections | 45002 | |
| Completeness [%] | 98.7 * | 96.6 |
| Redundancy | 5.8 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 7.3 | 21 * | ammonium sulfate, potassium phosphate, coenzyme A, pH 7.3, MICRODIALYSIS, temperature 294K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | ammonium sulfate | 1.9 (M) | |
| 2 | 1 | 1 | potassium phosphate | 0.1 (M) | |
| 3 | 1 | 1 | dithiothreitol | 1 (mM) | |
| 4 | 1 | 1 | CoA | 0.1 (mM) | |
| 5 | 1 | 1 | EDTA | 0.1 (mM) |
