1CPT
CRYSTAL STRUCTURE AND REFINEMENT OF CYTOCHROME P450-TERP AT 2.3 ANGSTROMS RESOLUTION
Experimental procedure
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 69.440, 69.440, 456.550 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 6.000 * - 2.300 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.980 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.200 * |
| Rmerge | 0.056 * |
| Total number of observations | 78920 * |
| Number of reflections | 24645 * |
| Completeness [%] | 69.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.8 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | P450 terp | ||
| 2 | 1 | drop | PEG12500 | 20 (%) | |
| 3 | 1 | drop | DTT | 15 (mM) | |
| 4 | 1 | drop | 80 (mM) | ||
| 5 | 1 | drop | 200 (mM) | ||
| 6 | 1 | reservoir | PEG12500 | 20 (%) | |
| 7 | 1 | reservoir | DTT | 15 (mM) | |
| 8 | 1 | reservoir | 80 (mM) | ||
| 9 | 1 | reservoir | 200 (mM) |
