1CPR

ST. LOUIS CYTOCHROME C' FROM THE PURPLE PHOTOTROPIC BACTERIUM, RHODOBACTER CAPSULATUS

Experimental procedure

Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Detector technology	IMAGE PLATE
Collection date	1995-12-19
Detector	FUJI
Spacegroup name	P 1 21 1
Unit cell lengths	39.680, 46.850, 40.750
Unit cell angles	90.00, 110.94, 90.00

Refinement procedure

Resolution	6.000 - 2.100
R-factor	0.184
Rwork	0.184
RMSD bond length	0.009
RMSD bond angle	20.020 *
Data reduction software	DENZO
Phasing software	X-PLOR (2.1)
Refinement software	X-PLOR (2.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *
High resolution limit [Å]	2.100 *	2.100 *
Rmerge	0.080	0.190 *
Total number of observations	27125 *
Number of reflections	6988
Completeness [%]	85.2	66.4 *
Redundancy	3.88

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG8000	18 (%)
3	1	reservoir	zinc acetate	0.2 (M)
4	1	reservoir	sodium cacodylate	0.1 (M)