1COZ
CTP:GLYCEROL-3-PHOSPHATE CYTIDYLYLTRANSFERASE FROM BACILLUS SUBTILIS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 277 |
| Detector technology | AREA DETECTOR |
| Detector | SDMS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.500, 61.400, 56.400 |
| Unit cell angles | 90.00, 113.10, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.25500 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 23.400 * |
| Data reduction software | SDMS |
| Data scaling software | SDMS |
| Phasing software | MLPHARE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.120 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.061 * | 0.125 * |
| Total number of observations | 45342 * | |
| Number of reflections | 16917 | |
| Completeness [%] | 88.6 | 83.8 * |
| Redundancy | 2.68 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.5 | 4 * | 100 MM TRIS PH 8.5 200 MM LITHIUM SULFATE 30% PEG 3350 (BAKER) |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6.3 (mg/ml) | |
| 2 | 1 | drop | Tris | 55 (mM) | |
| 3 | 1 | drop | EDTA | 0.63 (mM) | |
| 4 | 1 | drop | dithiothreitol | 0.63 (mM) | |
| 5 | 1 | drop | PEG3350 | 11 (%) | |
| 6 | 1 | drop | lithium sulfate | 75 (mM) | |
| 7 | 1 | reservoir | PEG3350 | 30 (%) | |
| 8 | 1 | reservoir | lithium sulfate | 200 (mM) | |
| 9 | 1 | reservoir | Tris | 100 (mM) |
