1CNM

ENHANCEMENT OF CATALYTIC EFFICIENCY OF PROTEINASE K THROUGH EXPOSURE TO ANHYDROUS ORGANIC SOLVENT AT 70 DEGREES CELSIUS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	285
Detector technology	IMAGE PLATE
Collection date	1998-09-01
Detector	MARRESEARCH
Spacegroup name	P 43 21 2
Unit cell lengths	68.320, 68.320, 108.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.200
R-factor	0.168 *
Rwork	0.168
R-free	0.23100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2prk
RMSD bond length	0.008
RMSD bond angle	0.025
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CCP4

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	3.000
High resolution limit [Å]	2.200	2.200
Rmerge	0.045	0.087
Total number of observations	89243 *
Number of reflections	12156
<I/σ(I)>	40.01	24.41
Completeness [%]	97.0	94
Redundancy	5.4	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	6.5 *	4 *	CRYSTALLIZED BY DISSOLVING THE 10%(W/V) OF LYOPHILIZED ENZYME IN 50MM TRIS.HCL, 1MM CACL2, PH 6.5. 25 MICRO LITRE DROPS OF THIS SOLUTION WERE EQUILIBRATED IN A MICRODIALYSIS SETUP AGAINST 1M NANO3 IN THE SAME BUFFER AT 4 DEGREE., pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	enzyme	10 (%(w/v))
2	1	1	Tris-HCl	50 (mM)
3	1	1		1 (mM)
4	1	2		1 (M)