1CMX

STRUCTURAL BASIS FOR THE SPECIFICITY OF UBIQUITIN C-TERMINAL HYDROLASES

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Collection date	1996-02-01
Detector	MARRESEARCH
Spacegroup name	H 3
Unit cell lengths	199.300, 199.300, 36.800
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.250
R-factor	0.248 *
Rwork	0.248
R-free	0.28500
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	25.900 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CCP4
Refinement software	X-PLOR (98.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.290
High resolution limit [Å]	2.250	2.250
Rmerge	0.037 *	0.202 *
Number of reflections	26106
<I/σ(I)>	14.1	4.3
Completeness [%]	99.0	96 *
Redundancy	4.3	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	4.5	21 *	16% PEG 6000 0.1 M SODIUM ACETATE PH 4.4, pH 4.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG6000	16 (%)
2	1	reservoir	sodium acetate	0.1 (M)