1CMV

HUMAN CYTOMEGALOVIRUS PROTEASE

Experimental procedure

Temperature [K]	296
Detector technology	IMAGE PLATE
Collection date	1995-11-24
Detector	MARRESEARCH
Spacegroup name	P 41 21 2
Unit cell lengths	76.800, 76.800, 172.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.270
R-factor	0.241
Rwork	0.241
R-free	0.31100 *
Structure solution method	MIR
RMSD bond length	0.009
RMSD bond angle	1.300 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.340
High resolution limit [Å]	2.270	2.260
Rmerge	0.080	0.411
Total number of observations	297289 *
Number of reflections	23705
<I/σ(I)>	17.38	1.81
Completeness [%]	94.5	75.7
Redundancy	12.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5		28-30% MONOMETHYL POLYETHYLENE GLYCOL 550 0.05-0.2 M NACL 10% GLYCEROL 50 MM HEPES AT PH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	mPEG550	28-33 (%)
2	1	reservoir		0.05-0.2 (M)
3	1	reservoir	glycerol	10 (%)
4	1	reservoir	HEPES	50 (mM)