1CMA
MET REPRESSOR/DNA COMPLEX + S-ADENOSYL-METHIONINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Detector technology | OSCILLATION CAMERA |
| Detector | ARNDT-WONACOTT |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 121.350, 121.350, 84.810 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.800 |
| R-factor | 0.22 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.680 |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.800 * |
| Rmerge | 0.074 * |
| Total number of observations | 33481 * |
| Number of reflections | 8856 |
| Completeness [%] | 97.7 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | pH 7.00, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 10 | 1 | 2 | NA CACODYLATE | ||
| 11 | 1 | 2 | NA AZIDE | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | NA CACODYLATE | ||
| 4 | 1 | 1 | NA AZIDE | ||
| 5 | 1 | 1 | CACL2 | ||
| 6 | 1 | 1 | NACL | ||
| 7 | 1 | 1 | SAM | ||
| 8 | 1 | 2 | WATER | ||
| 9 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | oligonucleotide | 5 (mg/ml) | |
| 2 | 1 | drop | protein | 9 (mg/ml) | |
| 3 | 1 | drop | calcium chloride | 22 (mM) | |
| 4 | 1 | drop | sodium cacodylate | 10 (mM) | |
| 5 | 1 | drop | sodium chloride | 6 (mM) | |
| 6 | 1 | drop | SAM | 1 (mg/ml) | |
| 7 | 1 | reservoir | sodium cacodylate | 10 (mM) | |
| 8 | 1 | reservoir | sodium azaid | 1 (mM) | |
| 9 | 1 | reservoir | 35 (%(v/v)) |
