1CM5

CRYSTAL STRUCTURE OF C418A,C419A MUTANT OF PFL FROM E.COLI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-02-01
Detector	MARRESEARCH
Spacegroup name	P 43 21 2
Unit cell lengths	159.059, 159.059, 159.784
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.300
R-factor	0.216
Rwork	0.216
R-free	0.25100
Structure solution method	MIR
RMSD bond length	0.007
RMSD bond angle	22.300 *
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	CNS (0.5)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.300
Rmerge	0.070
Total number of observations	469884 *
Number of reflections	87117
Completeness [%]	95.6
Redundancy	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	7.3		pH 7.3

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	10 (mg/ml)
10	1	2	EDTA	1 (mM)
11	1	2		3 (mM)
12	1	2		2 (mM)
13	1	2	PEG1000	24-30 (%(w/v))
2	1	1	Mops/NH3	25 (mM)
3	1	1		50 (mM)
4	1	1	dithiothreitol	10 (mM)
5	1	1		3 (mM)
6	1	1	PEG1000	16 (%(w/v))
7	1	2	Mops/NH3	25 (mM)
8	1	2		50 (mM)
9	1	2	dithiothreitol	1 (mM)