1CM3
HIS15ASP HPR FROM E. COLI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 273 |
| Detector technology | CCD |
| Collection date | 1994-06-21 |
| Detector | BRANDEIS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 25.570, 45.340, 27.620 |
| Unit cell angles | 90.00, 104.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.600 |
| R-factor | 0.191 |
| Rwork | 0.191 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | WILD TYPE E. COLI HPR |
| RMSD bond length | 0.001 |
| RMSD bond angle | 2.400 |
| Data reduction software | DENZO |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 10.000 |
| High resolution limit [Å] | 1.600 |
| Number of reflections | 10525 |
| Completeness [%] | 93.4 |
| Redundancy | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.4 * | 14 * | pH 5.4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | citrate phosphate | 0.1 (M) | |
| 2 | 1 | reservoir | ammonium sulfate | 20-25 (%sat) |
