1CLQ

CRYSTAL STRUCTURE OF A REPLICATION FORK DNA POLYMERASE EDITING COMPLEX AT 2.7 A RESOLUTION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	1998-10-15
Detector	ADSC
Spacegroup name	H 3
Unit cell lengths	209.740, 209.740, 114.250
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.000 - 2.700
R-factor	0.23
Rwork	0.230
R-free	0.28400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1waj
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	2.700
Rmerge	0.080 *
Total number of observations	51455 *
Number of reflections	23329 *
<I/σ(I)>	18.1
Completeness [%]	97.2
Redundancy	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	12 *	12% (W/V) PEG 350MME, 150 MM CACL2, 100 MM SODIUM CACODYLATE, PH 6.5, 12 DEGREES C, VAPOR DIFFUSION, HANGING DROP, temperature 285K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	PEG
2	1	1	CACL2
3	1	1	SODIUM CACODYLATE
4	1	2	PEG

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	mPEG350	12 (%(w/v))
2	1	reservoir	calcium chloride	150 (mM)
3	1	reservoir	sodium cacodylate	100 (mM)