1CLM

STRUCTURE OF PARAMECIUM TETRAURELIA CALMODULIN AT 1.8 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 1
Unit cell lengths	29.660, 53.790, 25.490
Unit cell angles	92.84, 97.02, 88.54

Refinement procedure

Resolution	10.000 * - 1.800
R-factor	0.21
Rwork	0.210
RMSD bond length	0.016
RMSD bond angle	3.000
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.800 *
Rmerge	0.070 *
Total number of observations	53853 *
Number of reflections	11458 *
Completeness [%]	85.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	calmodulin	3625 (mg/ml)
2	1	drop	distilled water		1 ml
3	1	drop		5 (mM)	1 ml
4	1	drop	MPD	55 (%(v/v))	2 ml
5	1	drop	cacodylate
6	1	reservoir	MPD	55 (%(v/v))
7	1	reservoir	cacodylate	0.05 (M)