1CL2

CYSTATHIONINE BETA-LYASE (CBL) FROM ESCHERICHIA COLI IN COMPLEX WITH AMINOETHOXYVINYLGLYCINE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1996-11-15
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	61.100, 154.700, 152.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.200
R-factor	0.164
Rwork	0.164
RMSD bond length	0.010
RMSD bond angle	1.890
Data reduction software	MOSFLM
Data scaling software	CCP4 ((AGROVATA)
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.300
High resolution limit [Å]	2.200	2.200
Rmerge	0.065
<I/σ(I)>		3.5
Completeness [%]	98.1	94.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.2		Laber, B., (1996) FEBS Lett., 379, 94. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	reservoir	KPi	50 (mM)
3	1	reservoir	PLP	0.010 (mM)