1CK7
GELATINASE A (FULL-LENGTH)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM02 |
| Synchrotron site | ESRF |
| Beamline | BM02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1997-10 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 121.320, 121.320, 345.110 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.000 - 2.800 |
| R-factor | 0.286 * |
| Rwork | 0.286 |
| R-free | 0.32700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gen PDB ENTRY 1FBL |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.024 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.000 | 2.980 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.117 * | 0.402 * |
| Total number of observations | 333608 * | |
| Number of reflections | 32057 | |
| <I/σ(I)> | 20.9 | 2 |
| Completeness [%] | 99.7 | 99.7 * |
| Redundancy | 10.2 | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.8 | 4 * | pH 7.8 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | reservoir | sodium glycine | 0.2 (M) | |
| 3 | 1 | reservoir | mPEG550 | 24-25 (%) | |
| 4 | 1 | reservoir | 0.28 (M) | ||
| 5 | 1 | reservoir | dithiothreitol | 0.01 (M) |
