1CJY

HUMAN CYTOSOLIC PHOSPHOLIPASE A2

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	1998-03-01
Detector	ADSC
Wavelength(s)	1.2, 1.6496
Spacegroup name	P 21 21 2
Unit cell lengths	153.590, 95.490, 139.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	12.000 - 2.500
R-factor	0.229
Rwork	0.229
R-free	0.29800
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.940
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	12.000
High resolution limit [Å]	2.500
Rmerge	0.064 *	0.300 *
Total number of observations	271686 *
Number of reflections	66223
<I/σ(I)>	18.2
Completeness [%]	93.3	87.9 *
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.2 *	18 *	pH 8.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	MES	0.1 (M)
2	1	drop	spermidine	30 (mM)
3	1	drop	PEG1000	8 (%)
4	1	drop	DMSO	13 (%)
5	1	drop	protain	12 (mg/ml)