1CJ0
CRYSTAL STRUCTURE OF RABBIT CYTOSOLIC SERINE HYDROXYMETHYLTRANSFERASE AT 2.8 ANGSTROM RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 116.380, 116.380, 165.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| Rwork | 0.214 |
| R-free | 0.28000 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.016 |
| RMSD bond angle | 23.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 35.000 |
| High resolution limit [Å] | 2.800 |
| Rmerge | 0.173 |
| Number of reflections | 28327 |
| <I/σ(I)> | 10.9 |
| Completeness [%] | 100.0 |
| Redundancy | 19 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 20MM K2HPO4, KH2PO4, 25MM POTASIUM MES OR SODIUM HEPES, PH 7.0 0.2 MM 2-MERCAPTOETHANOL, 3% PEG 400, pH 6.0, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | 20 (mM) | ||
| 2 | 1 | 1 | KMES | 25 (mM) | or sodium HEPES |
| 3 | 1 | 1 | EDTA | 0.2 (mM) | |
| 4 | 1 | 1 | 2-mercaptoethanol | 5 (mM) | |
| 5 | 1 | 1 | PEG4000 | 3 (%) |
