1CIW
PEANUT LECTIN COMPLEXED WITH N-ACETYLLACTOSAMINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 129.380, 126.922, 76.065 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.700 |
| R-factor | 0.194 * |
| Rwork | 0.194 |
| R-free | 0.24400 |
| Structure solution method | OTHER |
| Starting model (for MR) | PDB2PEL.ENT WITHOUT SUGAR AND WATER |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.100 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 99.000 |
| High resolution limit [Å] | 2.700 |
| Rmerge | 0.124 |
| Total number of observations | 185971 * |
| Number of reflections | 34005 |
| Completeness [%] | 97.8 |
| Redundancy | 5.46 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | pH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 10 | 1 | reservoir | sugar | 1.5-10 (mM) | |
| 11 | 1 | reservoir | sodium phosphate | 0.05 (M) | |
| 2 | 1 | drop | sodium phosphate | 0.05 (M) | |
| 3 | 1 | drop | 0.2 (M) | ||
| 4 | 1 | drop | sodium azide | 0.02 (%) | |
| 5 | 1 | drop | sugar | 1.5-10 (mM) | |
| 6 | 1 | drop | PEG8000 | 12 (%(w/v)) | |
| 7 | 1 | reservoir | PEG8000 | 40 (%(w/v)) | |
| 8 | 1 | reservoir | 0.2 (M) | ||
| 9 | 1 | reservoir | sodium azide | 0.02 (%) |
