1CIV
CHLOROPLAST NADP-DEPENDENT MALATE DEHYDROGENASE FROM FLAVERIA BIDENTIS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM02 |
| Synchrotron site | ESRF |
| Beamline | BM02 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-09-01 |
| Detector | RIGAKU |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 147.800, 147.800, 64.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.800 |
| R-factor | 0.205 * |
| Rwork | 0.205 |
| R-free | 0.28400 |
| Structure solution method | SIRAS |
| Starting model (for MR) | 4mdh |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.740 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | X-PLOR (3.84) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.800 |
| Rmerge | 0.132 |
| Total number of observations | 38553 * |
| Number of reflections | 20335 |
| Completeness [%] | 98.3 |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.5 | 291 * | SEE MACPHERSON ET AL. (1998) ACTA CRYST, D54, 654 - 656, pH 7.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | ammonium sulfate | 1.5 (M) | |
| 2 | 1 | 1 | HEPES | 0.1 (M) | |
| 3 | 1 | 1 | glycerol | 12 (%) |
