1CIA

REPLACEMENT OF CATALYTIC HISTIDINE-195 OF CHLORAMPHENICOL ACETYLTRANSFERASE: EVIDENCE FOR A GENERAL BASE ROLE FOR GLUTAMATE

Experimental procedure

Spacegroup name	H 3 2
Unit cell lengths	107.640, 107.640, 124.130
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 2.500
R-factor	0.131
RMSD bond length	0.020
RMSD bond angle	0.044 *
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.500 *
Rmerge	0.043 *	0.103 *
Total number of observations	23710 *
Number of reflections	9485 *
Completeness [%]	97.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	6.3 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	5 (mg/ml)
2	1	1	MES	10 (mM)
3	1	2	MPD	2-4 (%)
4	1	2	MES	10 (mM)
5	1	2	chloramphenicol	1 (mM)
6	1	2	hexaamminecobalt(III)chloride	0.5 (mM)
7	1	2	2-mercaptoethanol	5 (mM)