1CIA
REPLACEMENT OF CATALYTIC HISTIDINE-195 OF CHLORAMPHENICOL ACETYLTRANSFERASE: EVIDENCE FOR A GENERAL BASE ROLE FOR GLUTAMATE
Experimental procedure
Spacegroup name | H 3 2 |
Unit cell lengths | 107.640, 107.640, 124.130 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 6.000 - 2.500 |
R-factor | 0.131 |
RMSD bond length | 0.020 |
RMSD bond angle | 0.044 * |
Refinement software | PROLSQ |
Data quality characteristics
Overall | Outer shell | |
High resolution limit [Å] | 2.500 * | |
Rmerge | 0.043 * | 0.103 * |
Total number of observations | 23710 * | |
Number of reflections | 9485 * | |
Completeness [%] | 97.0 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microdialysis * | 6.3 * | 4 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 5 (mg/ml) | |
2 | 1 | 1 | MES | 10 (mM) | |
3 | 1 | 2 | MPD | 2-4 (%) | |
4 | 1 | 2 | MES | 10 (mM) | |
5 | 1 | 2 | chloramphenicol | 1 (mM) | |
6 | 1 | 2 | hexaamminecobalt(III)chloride | 0.5 (mM) | |
7 | 1 | 2 | 2-mercaptoethanol | 5 (mM) |