1CHK
STREPTOMYCES N174 CHITOSANASE PH5.5 298K
Experimental procedure
Temperature [K] | 298 |
Detector technology | AREA DETECTOR |
Collection date | 1993-09-29 |
Detector | XUONG-HAMLIN MULTIWIRE |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.300, 59.300, 85.400 |
Unit cell angles | 90.00, 96.20, 90.00 |
Refinement procedure
Resolution | 5.000 - 2.400 |
R-factor | 0.1809 * |
Rwork | 0.181 |
R-free | 0.17480 * |
RMSD bond length | 0.016 * |
RMSD bond angle | 3.118 * |
Data reduction software | SDMS |
Data scaling software | SDMS |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.400 |
Rmerge | 0.088 |
Total number of observations | 121478 * |
Number of reflections | 21903 |
Completeness [%] | 98.7 * |
Redundancy | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5.5 | Marcotte, E., (1993) J.Mol.Biol., 232, 995. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 30 (mg/ml) | |
2 | 1 | drop | sodium acetate | 20 (mM) | |
3 | 1 | drop | potassium phosphate | 0.5 (M) | |
4 | 1 | drop | PEG8000 | 20 (%(w/v)) | |
5 | 1 | reservoir | potassium phosphate | 0.5 (M) | |
6 | 1 | reservoir | PEG8000 | 20 (%(w/v)) |