1CH0

RNASE T1 VARIANT WITH ALTERED GUANINE BINDING SEGMENT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ELLIOTT GX-21
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1998-08
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	39.820, 48.850, 158.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.600 - 2.300
R-factor	0.241 *
Rwork	0.198
R-free	0.19800 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rnt
RMSD bond length	0.006
RMSD bond angle	23.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.600	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.115 *	0.396 *
Number of reflections	14434
<I/σ(I)>	9.04	2.8
Completeness [%]	97.7	98.1
Redundancy	3.14

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.7	18 *	HANGING DROP CONTAINING 2.5 MG/ML RNASE T1, 2.5 MG/ML 2GMP, 15% PEG MONOMETHYLETHER 5000, 50 MM TRIS/HCL (PH7.7), 50 MM MGCL2, 50 MM CACL2 AGAINST 30 % PEG MONOMETHYLETHER 5000, 100 MM TRIS/HCL (PH7.7), 100 MM MGCL2, 100 MM CACL2

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)	in water
2	1	drop	2'-GMP	10 (mg/ml)	in water
3	1	reservoir	mPEG5000	30 (%(w/v))
4	1	reservoir	Tris-HCl	100 (mM)	pH7.7
5	1	reservoir		100 (mM)
6	1	reservoir		100 (mM)