1CGZ

CHALCONE SYNTHASE FROM ALFALFA COMPLEXED WITH RESVERATROL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	MACSCIENCE
Temperature [K]	105
Detector technology	IMAGE PLATE
Collection date	1998-06
Detector	MAC Science DIP-2000
Spacegroup name	P 32 2 1
Unit cell lengths	97.657, 97.657, 65.359
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	85.000 - 1.700
R-factor	0.157 *
Rwork	0.171
R-free	0.21700
Structure solution method	OTHER
Starting model (for MR)	1bi5
RMSD bond length	0.011
RMSD bond angle	2.350
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CCP4 ((ARPP))
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	84.500	1.740
High resolution limit [Å]	1.700	1.700
Rmerge	0.048 *
Number of reflections	34919
<I/σ(I)>	13	12.4
Completeness [%]	87.6	36.2
Redundancy	1.35	0.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5	4 *	pH 6.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25 (mg/ml)
2	1	reservoir	ammonium sulfate	2.2-2.4 (M)
3	1	reservoir	PIPES	0.1 (M)