1CGU

CATALYTIC CENTER OF CYCLODEXTRIN GLYCOSYLTRANSFERASE DERIVED FROM X-RAY STRUCTURE ANALYSIS COMBINED WITH SITE-DIRECTED MUTAGENESIS

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	94.100, 105.000, 113.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.500
R-factor	0.187
Rwork	0.187
RMSD bond length	0.018
RMSD bond angle	3.600
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.500 *
Rmerge	0.121 *
Completeness [%]	76.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein solution	50 (%(v/v))
2	1	drop	CGTase	7-15 (mg/ml)
3	1	drop	Tris-HCl	50 (mM)
4	1	drop	PEG1500	0.5 (%)
5	1	reservoir	ammonium sulphate	0.9-1.0 (M)
6	1	reservoir	CGTase	7-15 (mg/ml)
7	1	reservoir	Tris-HCl	50 (mM)
8	1	reservoir	PEG1500	0.5 (%)