1CGQ
MACROPHAGE MIGRATION INHIBITORY FACTOR (MIF) WITH ALANINE INSERTED BETWEEN PRO-1 AND MET-2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 133 |
Collection date | 1997-12-01 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.810, 67.870, 88.770 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
Rwork | 0.234 |
R-free | 0.27500 |
Structure solution method | OTHER |
RMSD bond length | 0.010 |
RMSD bond angle | 1.510 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.240 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.078 * | 0.094 * |
Total number of observations | 115296 * | |
Number of reflections | 27733 * | |
<I/σ(I)> | 6 | |
Completeness [%] | 98.2 | 96.8 |
Redundancy | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 | pH 7.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10-15 (mg/ml) | |
2 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
3 | 1 | reservoir | HEPES | 0.1 (M) | |
4 | 1 | reservoir | PEG400 | 2 (%) |