1CGF
CRYSTAL STRUCTURES OF RECOMBINANT 19-KDA HUMAN FIBROBLAST COLLAGENASE COMPLEXED TO ITSELF
Experimental procedure
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.000, 50.500, 48.000 |
| Unit cell angles | 90.00, 100.00, 90.00 |
Refinement procedure
| Resolution | 7.000 - 2.100 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 2.060 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.100 * |
| Rmerge | 0.062 * |
| Number of reflections | 17088 * |
| Completeness [%] | 86.7 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | sodium formate | 2 (M) | |
| 2 | 1 | 1 | calcium chloride | 1 (mM) | |
| 3 | 1 | 1 | zinc chloride | 0.05 (M) | |
| 4 | 1 | 1 | Tris-HCl | 0.1 (M) |
