1CD1

CD1(MOUSE) ANTIGEN PRESENTING MOLECULE

Experimental procedure

Source type	ROTATING ANODE
Source details	ELLIOTT GX-18
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1994-09-02
Detector	ELLIOT
Spacegroup name	P 1 21 1
Unit cell lengths	59.340, 76.200, 103.400
Unit cell angles	90.00, 102.29, 90.00

Refinement procedure

Resolution	10.000 - 2.670
R-factor	0.192
Rwork	0.192
R-free	0.33000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	FCRN HLA-B27
RMSD bond length	0.013
RMSD bond angle	27.000 *
Data reduction software	XENGEN
Data scaling software	XENGEN
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.142	2.661
High resolution limit [Å]	2.557	2.557
Rmerge	0.095 *
Total number of observations	57464 *
Number of reflections	22974
<I/σ(I)>	7.795	1.3
Completeness [%]	75.0	21
Redundancy	1.93	1.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.5		6 MG/ML PROTEIN 2.0M (NH4)2SO4 100 MM SODIUM CACODYLATE, 20MM EDTA, PH 6.5 5 UL PROTEIN + 2.5 UL RESERVOIR SITTING DROP, vapor diffusion - sitting drop

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	mCD1d1	6 (mg/ml)
2	1	2	ammonium sulfate	2.0 (M)
3	1	2	sodium citrate	0.15 (M)
4	1	2		2 (mM)