1CBW
BOVINE CHYMOTRYPSIN COMPLEXED TO BPTI
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 295 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1992-01 |
| Detector | ENRAF-NONIUS FAST |
| Spacegroup name | P 61 |
| Unit cell lengths | 101.600, 101.600, 205.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.600 |
| R-factor | 0.192 |
| Rwork | 0.192 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTTY 5CHA + 2PTC |
| RMSD bond length | 0.015 |
| RMSD bond angle | 26.470 * |
| Data reduction software | PROCOR |
| Data scaling software | PROCOR |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.087 | 0.120 |
| Number of reflections | 32244 | |
| <I/σ(I)> | 10 | 1.5 |
| Completeness [%] | 89.0 | 55 |
| Redundancy | 3 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.5 | pH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | complex | 20 (mg/ml) | |
| 2 | 1 | drop | MPD | 0.5 (%(v/v)) | |
| 3 | 1 | drop | ammonium sulfate | 50 (%(v/v)) | |
| 4 | 1 | drop | Tris-HCl | 80 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | 50 (%(v/v)) | |
| 6 | 1 | reservoir | Tris-HCl | 80 (mM) |
