1CBF

THE X-RAY STRUCTURE OF A COBALAMIN BIOSYNTHETIC ENZYME, COBALT PRECORRIN-4 METHYLTRANSFERASE, CBIF

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.5
Synchrotron site	SRS
Beamline	PX9.5
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	1997-03
Detector	MARRESEARCH
Spacegroup name	P 31 2 1
Unit cell lengths	80.700, 80.700, 109.400
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.400
R-factor	0.204 *
Rwork	0.195
R-free	0.25200 *
Structure solution method	MULTIPLE ISOMORPHOUS REPLACEMENT
RMSD bond length	0.014 *
RMSD bond angle	2.300 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.440
High resolution limit [Å]	2.400	2.400
Rmerge	0.039	0.122
Number of reflections	16587
<I/σ(I)>	21.3	10
Completeness [%]	99.8 *	100
Redundancy	5	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5.6 *		pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	sodium acetate	20 (mM)
2	1	1		100 (mM)
3	1	2	protein	16-20 (mg/ml)