1C9W
CHO REDUCTASE WITH NADP+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-07-16 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 84.282, 84.282, 120.539 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 2.400 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| R-free | 0.24400 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.198 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 2.490 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.064 | 0.253 |
| Total number of observations | 420251 * | |
| Number of reflections | 19882 | |
| <I/σ(I)> | 14.5 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.1 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | 298 | drop consists of equal volume of protein and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | EDTA | 1 (mM) | |
| 3 | 1 | drop | PIPES | 10 (mM) | |
| 4 | 1 | drop | beta-mercaptoethanol | 1 (mM) | |
| 5 | 1 | drop | NADP+ | 49 (mM) | 0.02ml |
| 6 | 1 | reservoir | PEG4000 | 0.2 (M) | |
| 7 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
| 8 | 1 | reservoir | 2,5-hexanediol | 0.4 (%(v/v)) |
