1C9B

CRYSTAL STRUCTURE OF A HUMAN TBP CORE DOMAIN-HUMAN TFIIB CORE DOMAIN COMPLEX BOUND TO AN EXTENDED, MODIFIED ADENOVIRAL MAJOR LATE PROMOTER (ADMLP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F2
Synchrotron site	CHESS
Beamline	F2
Temperature [K]	100
Detector technology	CCD
Collection date	1998-05-15
Detector	ADSC QUANTUM 4
Spacegroup name	P 1 21 1
Unit cell lengths	118.450, 122.298, 140.217
Unit cell angles	90.00, 113.08, 90.00

Refinement procedure

Resolution	50.000 - 2.650
R-factor	0.231
Rwork	0.229
R-free	0.26000
RMSD bond length	0.013
RMSD bond angle	1.580 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.710
High resolution limit [Å]	2.650	2.650
Rmerge	0.057	0.341
Number of reflections	101892
<I/σ(I)>	16
Completeness [%]	97.5	85.4
Redundancy	3.98	2.25

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6 *	4 *	PEG 8000, TRIS.HCL, AMMONIUM ACETATE, MAGNESIUM CHLORIDE, POTASSIUM CHLORIDE, SODIUM CITRATE, DTT, GLYCEROL, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	TRIS.HCL
10	1	2	PEG 8000
2	1	1	AMMONIUM ACETATE
3	1	1	MGCL2
4	1	1	KCL
5	1	1	SODIUM CITRATE
6	1	1	DTT
7	1	1	GLYCEROL
8	1	1	PEG 8000
9	1	2	GLYCEROL

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG8000	4 (%(w/v))
3	1	reservoir	Tris-HCl	50 (mM)
4	1	reservoir		50 (mM)
5	1	reservoir	sodium citrate	100 (mM)
6	1	reservoir	glycerol	10 (%)