1C8V
CRYSTAL STRUCTURE OF THE COMPLEX OF BACTERIAL TRYPTOPHAN SYNTHASE WITH THE TRANSITION STATE ANALOGUE INHIBITOR 4-(2-HYDROXYPHENYLTHIO)-BUTYLPHOSPHONIC ACID
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 140 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-06-27 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 183.800, 60.770, 68.150 |
| Unit cell angles | 90.00, 94.38, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.200 |
| R-factor | 0.212 |
| Rwork | 0.208 |
| R-free | 0.26800 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 24.640 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.200 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.077 | 0.241 |
| Number of reflections | 35708 | |
| <I/σ(I)> | 10.35 | |
| Completeness [%] | 93.5 | 79 |
| Redundancy | 3.3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.8 | 295 | pH was adjusted with NaOH * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-10 (mg/ml) | |
| 2 | 1 | drop | inhibitor | 1 (mM) | |
| 3 | 1 | reservoir | Bicine | 50 (mM) | |
| 4 | 1 | reservoir | NaEDTA | 1 (mM) | |
| 5 | 1 | reservoir | spermine | 0.8-1.5 (mM) | |
| 6 | 1 | reservoir | PEG4000 | 12 (%) |
