1C8H

CANINE PARVOVIRUS STRAIN D EMPTY CAPSID STRUCTURE AT PH 5.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	1999-01-15
Detector	ADSC QUANTUM 4
Spacegroup name	P 1
Unit cell lengths	254.493, 253.601, 452.044
Unit cell angles	77.48, 74.91, 69.27

Refinement procedure

Resolution	9.000 - 3.500
R-factor	0.26
RMSD bond length	0.011
RMSD bond angle	1.688
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	GLRF
Refinement software	CNS (0.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.590
High resolution limit [Å]	3.500	3.500
Rmerge	0.095	0.121
Number of reflections	812178
<I/σ(I)>	4.4
Completeness [%]	63.7	40.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.5 *	19 *	0.75-1.5% PEG8000, 8 mM CaCl2, 20 mM Bis-Tris pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	virus	10 (mg/ml)
2	1	drop	Bis-Tris	20 (mM)
3	1	drop	Tris-HCl	20 (mM)
4	1	reservoir	PEG8000	0.75-1.50 (%)
5	1	reservoir		8 (mM)	or 5mM EDTA