1C8F

FELINE PANLEUKOPENIA VIRUS EMPTY CAPSID STRUCTURE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	277
Detector technology	OSCILLATION CAMERA
Collection date	1992-01-15
Wavelength(s)	0.9, 1.4
Spacegroup name	P 21 21 21
Unit cell lengths	380.120, 379.250, 350.820
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	9.000 - 3.000
R-factor	0.285
Rwork	0.285
RMSD bond length	0.009
RMSD bond angle	1.589
Phasing software	GLRF
Refinement software	CNS (0.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.800	3.200
High resolution limit [Å]	2.700	3.000
Rmerge	0.146
Number of reflections	547268
<I/σ(I)>	11.2
Completeness [%]	59.6	35

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.5	19 *	0.75-1.5% PEG8000, 8 mM CaCl2, 20 mM Tris pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K
1	Vapor diffusion, sitting drop *	7.5	19 *	0.75-1.5% PEG8000, 8 mM CaCl2, 20 mM Tris pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	virus	10 (mg/ml)
2	1	drop	Bis-Tris	20 (mM)
3	1	drop	Tris-HCl	20 (mM)
4	1	reservoir	PEG8000	0.75-1.50 (%)
5	1	reservoir		8 (mM or 5 mM EDTA)