1C8E
FELINE PANLEUKOPENIA VIRUS EMPTY CAPSID STRUCTURE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-01-15 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 254.154, 253.962, 380.288 |
| Unit cell angles | 90.00, 92.83, 90.00 |
Refinement procedure
| Resolution | 9.000 - 3.000 |
| R-factor | 0.288 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.680 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | GLRF |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.092 | 0.221 |
| Number of reflections | 810220 | |
| <I/σ(I)> | 12.3 | |
| Completeness [%] | 89.2 | 47.3 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.5 * | 19 * | 0.75-1.5% PEG8000, 5 mM EDTA, 20 mM BIS-Tris pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 0.75-1.50 (%(w/v)) | |
| 2 | 1 | reservoir | 8 (mM) | or 5mM EDTA | |
| 3 | 1 | drop | virus | 10 (mg/ml) | |
| 4 | 1 | drop | Bis-Tris | 20 (mM) | |
| 5 | 1 | drop | Tris-HCl | 20 (mM) |
