1C7Y
E.COLI RUVA-HOLLIDAY JUNCTION COMPLEX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6B |
Synchrotron site | Photon Factory |
Beamline | BL-6B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-12-15 |
Spacegroup name | I 4 3 2 |
Unit cell lengths | 158.650, 158.650, 158.650 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.800 - 3.100 |
R-factor | 0.25 |
Rwork | 0.249 |
R-free | 0.27900 |
RMSD bond length | 0.011 |
RMSD bond angle | 20.400 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.054 | 0.275 |
Total number of observations | 143269 * | |
Number of reflections | 7136 | |
<I/σ(I)> | 47 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 13.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 20 * | ammonium sulfate, sodium chloride, glycerol, MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MES | ||
2 | 1 | 1 | glycerol | ||
3 | 1 | 1 | NaCl | ||
4 | 1 | 1 | (NH4)2SO4 | ||
5 | 1 | 2 | glycerol | ||
6 | 1 | 2 | NaCl | ||
7 | 1 | 2 | (NH4)2SO4 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | Mes/NaOH | 0.1 (M) | |
2 | 1 | reservoir | ammonium sulfate | 2.0-2.2 (M) | |
3 | 1 | reservoir | glycerol | 5 (%) |