1C7P

CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME WITH FOUR EXTRA RESIDUES (EAEA) AT THE N-TERMINAL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-18B
Synchrotron site	Photon Factory
Beamline	BL-18B
Temperature [K]	283
Detector technology	DIFFRACTOMETER
Collection date	1998-12-15
Detector	WEISSENBERG
Spacegroup name	P 61 2 2
Unit cell lengths	91.080, 91.080, 92.730
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	5.000 - 2.400
R-factor	0.197
RMSD bond length	0.011
RMSD bond angle	1.648
Data reduction software	DENZO
Phasing software	AMoRE
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.400
Rmerge	0.044
Total number of observations	72761 *
Number of reflections	9367
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	10 *	equal volume of protein and reservoir solution were used for the drop *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	cadmium chloride	0.1 (M)
3	1	reservoir	sodium acetate	0.1 (M)
4	1	reservoir	PEG400	30 (%(v/v))